Hi,
Im trying to do my lab work.
I have 1mL of nicotine (possibly 2mL) and I need to make some dilutions to make a calibration curve on UV-VIS 1800.
I have been told to mix the nicotine with HPLC grade water but im not sure how. Due to the quantity of nicotine, i know it will require a serial dilution but im not sure on any of the numbers
any help will be appreciated,
thanks!
EDIT: The cuvettes are 3mL on the UV-VIS and I need roughly (this is adaptable) the following concentration. Also, the concentration needs to cover 20 - 24 mg/ml nicotine as the highest really. (its being compared against e-liquids)
Its impossible! We need more information like the initial concentration of the standard in mg/mL. For 3 mL cuvette you will probably use 5.0 or 10.0 mL glass volumetrics. Thus,
Serial Concentration (mg/mL) = y initial concentration (mg/mL) x [ mL volume/5.0 mL Volumetric]
The initial concentration of the nicotine standard is 98mg/mL I believe? Does that help at all. The problem I am having it with the quantity of nicotine as it is so small and I cant have more.
I have plentiful HLPC grade water though
You would have to use a micropipette, which usually delivers volumes in uL (1/1000 mL). Thus 100 uL is 0.10 mL. As an example;
98 mL/mL x 0.10 mL/5.0 mL = 1.96 mg/mL
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