I have extracted RNA from avian blood samples using Trizol but I am not sure about the bands on gel. I have used 1% agarose gel and 1x TBE buffer and electrophoresis was carried out at 80volts for 35minutes. I have attached the picture of gel. I am not sure whether the top bands are due to DNA contamination or RNA may be I have not run it for appropriate time.
HI,
Based on the gel the 18s and 28s bands are visible. Pls run a little longer for proper resolution of bands.
I think that you have contamination of DNA. Please use a small aliquot of sample and perform DNAse treatment. Just to confirm.
hope it helps.
Santosh
The bands are there but the RNA quality might not be high. Also always continue with cDNA conversion even when you don't get proper bands. Check the integrity by amplifying with a housekeeping gene.
It is hard to tell but your rRNA bands seems intact and hence the quality seems good. Generally we run on 2% agarose for clear differentiation of 28s and 16s rRNA. mRNA will appear as a smear in a gel.
Shajahan
You're welcome. Also check this website for some useful info.
https://www.thermofisher.com/uk/en/home/references/ambion-tech-support/rna-isolation/tech-notes/is-your-rna-intact.html
Cheers,
Shajahan
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