I have read it somewhere in an article that avian defensin mRNAs have been found abundantly in the bone marrow and their proteins in heterophil granules, so I was wondering if someone is working with avian blood samples is there any possibility of getting defensin's RNA from blood?
Hello
i don't worked with avian blood but the discussion and article here will help you
Good Luck
http://www.tandfonline.com/doi/pdf/10.1080/00071668.2014.888397
https://www.researchgate.net/post/Can_anyone_help_with_RNA_isolation_from_avian_blood_stored_in_RNAlater
Would be interested to know how you get on. Not sure how abundant the transcripts for defensins will be as they are not the easiest to get good expression values for from tissues. Getting rid of the erythrocytes and thrombocytes may help though is not so easy. Alternatively if you are interested in the expression specifically by heterophils -you could isolate these over a discontinuous histopaque/ficoll gradient. Good luck
I am using Trizol reagent for RNA isolation and i have isolated RNA easily there was no such issue of erythrocytes or thrombocytes. The thing I am worried about is that most of the defensin's transcripts have been isolated from other tissues not blood now I just hope to get altleast some of the transcripts from blood as well.
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