I would like to insert my cDNA of interest, Xdh, in a plasmidic vector in order to transect my cells. I have already designed a pair of primers for the adding of the restriction sites in my cDNA. i don't have cDNA or mRNA of my gene so I have to extract fresh from a tissue sample. My doubt is this: can I extract directly DNA and use it as a template for the amplification with the primers leading the RE sites avoiding to design another pair of primers for the mRNA? 

The question is if I have to start from a mRNA pool and make a retro-transcription in cDNA, and use this as a template or I can extract directly DNA?

Thank you so much

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