Gianmichele Massimo

5 Questions 40 Answers 0 Followers

Questions related from Gianmichele Massimo

4000bp cDNA PCR amplification problems...Is there anyone that can give an opinion?

Dear All I have to make a PCR with my designed primers bringing the restriction enzyme site at the 3' and 5'. However I am having problem as well for getting cDNA band for my 4000bp cDNA. I have...

11 June 2015 3,351 28 View

Can anyone tell me a reason for which I am not able to get cDNA plasmid vector from bacteria?

As in the past I have transformed my bacteria with a cDNA plasmid vector, and I seed them into dishes. First thing that I noted that bacteria grew just in the first 2/3 of the dishes, where I...

16 April 2015 9,292 5 View

Does anyone have a small aliquot of the empty pCMV6-AC-GFP plasmid?

Is there someone who can very kindly give me a special gift, a few ul of the pCMV6-AC-GFP plasmid? I would like to save some money, and just because I only need it for just one time, I would like...

13 March 2015 6,778 3 View

Can someone give me a suggestion on the cloning procedures?

I would like to insert my cDNA of interest, Xdh, in a plasmidic vector in order to transect my cells. I have already designed a pair of primers for the adding of the restriction sites in my cDNA....

12 March 2015 1,860 4 View

Hek-293 stable cell line problems?

I am having a problem with the selection of my HEK-293 cells. I started the selection with G418 two months ago and I still have in my flask untransfected cells that don't die. What can I do? Try...

21 June 2013 1,990 19 View