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Questions related from Gianmichele Massimo
Dear All I have to make a PCR with my designed primers bringing the restriction enzyme site at the 3' and 5'. However I am having problem as well for getting cDNA band for my 4000bp cDNA. I have...
11 June 2015 3,354 28 View
As in the past I have transformed my bacteria with a cDNA plasmid vector, and I seed them into dishes. First thing that I noted that bacteria grew just in the first 2/3 of the dishes, where I...
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Is there someone who can very kindly give me a special gift, a few ul of the pCMV6-AC-GFP plasmid? I would like to save some money, and just because I only need it for just one time, I would like...
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I would like to insert my cDNA of interest, Xdh, in a plasmidic vector in order to transect my cells. I have already designed a pair of primers for the adding of the restriction sites in my cDNA....
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I am having a problem with the selection of my HEK-293 cells. I started the selection with G418 two months ago and I still have in my flask untransfected cells that don't die. What can I do? Try...
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