I am going to perform transfection of plasmids. I have got them from Addgene. 1st time I extracted plasmid using a sample mini prep kit from a company the quality was good. But next time I used Pure yield mini prep kit for extraction of plasmids. I had already made 12 preps but all of the results were very bad. It looks like there is no plasmid. I can not understand what is the problem. I need abundant amount of plasmids for transfection. Can someone suggest a solution?
Usually mini preps yields are not enough to have a good transfection, in my experience you should try a midi prep instead. For transfection into HeLa cells I used a midi prep kit from Axygen, and the amount I got was more than enough to repeat my transfection a few times. Good luck!
use PureLink kits (Invitrogen)- available at mini through giga scales, and of different extent of purity. for transfections endotoxin-free option is the best
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