A.K.M. Moyeenul Huq

20 Questions 66 Answers 0 Followers

Questions related from A.K.M. Moyeenul Huq

How can a mixture of crystals obtained from plant extract can be separated?

I am isolating compounds from plant. i have got 2 types of crystals,one is needle like and another cubic in shape in a vial. now from here how can i separate them? any technique??

03 April 2015 5,695 6 View

Is a cDNA synthesis kit for realtime PCR compatible with conventional end point RT PCR?

I have been synthesizing cDNA using QuantiTech Rev Transcrp (Qiagen) kit to see the expression of TFF1 gene. In the label of the kit box it says that this kit synthesize cDNA which is specific for...

29 December 2014 2,561 8 View

Can anyone assist with identifying Plasmids from Glycerol stock?

I have three Plasmids in glycerol stock. I labelled them and kept in -80 freezer for 6-7 months. Now when I wanted to take them out from the freezer I found the the labels disappeared. Now is...

18 August 2014 2,361 12 View

What are the effects of the concentration of crude extracts on bioactivity?

I have done a MTT cell proliferation assay on a cell line with 6 different concentrations of a plant crude extract, and replicated 4 times. In most cases, I found the activity was found in the...

01 June 2014 6,850 12 View

How to prepare a primer solution?

I have primers from Integrated DNA Technologies in lyophilized form. The amount of Oligo is 25.5 nMoles = 0.16 mg. Now how should I dissolve it? Which solvent should I use? And how much of it?

25 March 2014 6,687 11 View

Can anyone advise on a cell culture problem?

I am culturing MCF-7 cancer cell line in RPMI with phenol red and without phenol red for my experiment. 10% FBS is used. The passage is 42. For the last couple of passages I see that the cells are...

18 January 2014 4,729 10 View

Can anyone help with making RNase free?

If I don't have DEPC/DEPC treated water, what alternative can I use to make my working pipette, tubes, tips RNase free ? And how?

18 December 2013 1,942 8 View

Can someone advise on plasmid purity and quality?

I am going to perform transfection of plasmids. I have got them from Addgene. 1st time I extracted plasmid using a sample mini prep kit from a company the quality was good. But next time I used...

06 July 2013 4,145 2 View

GC-MS for crude extracts

Can we run GC-MS for crude hexane, DCM and methanol extracts to characterize the possible type of compounds present in those extracts? If so then how can we prepare the sample to GC-MS? What...

10 May 2013 2,474 9 View

RNA ladder

I want to buy an RNA ladder. How do I know which size I need to buy (high range or low range)? What are the things I need to consider?

09 May 2013 8,925 3 View

How to calculate the volume of medium containing the cell (MCF7 cell line) needed to seed 3000 cell/well in 96 well plate for MTT assay?

I want to perform an MTT assay for a cell proliferation study. After culturing the cells, when we need to seed the cells in a 96 well plate then how would I calculate the volume of cell containing...

22 March 2013 6,119 5 View

What is the purpose of using Standard compounds in TLC with crude extract?

How do Standard compounds help in TLC with crude extracts? Specifically when we look for flavonois we use flavonoid standard.

22 January 2013 1,886 3 View

Can I use C18 HPLC column for non polar extracts? If so, then how to prepare the sample for that and what kind of solvents can use as moble phase?

Is ii possible to run HPLC analytical crude extract of Hexane and DCM using C18 column? How to prepare extract sample and what will be the choice of solvent for mobile phase?

12 January 2013 5,142 10 View

Can anyone suggest me any software/web by which I can get the Chemical structure of any compound from the compound name?

I ned to get the structure of some compounds (Phyto). I have the names only but need the structure. any software/website to convert the chemical name to its structure?

26 December 2012 5,974 11 View

For Water extract what mobile phase can I use for HPLC? How should I prepare my sample?

I want to run HPLC for my Water extract. For my plant no paper found to chose a solvent system for HPLC.

24 December 2012 9,574 8 View

Problem with HPLC: its pressure is not getting stable. How can I solve this problem? What are the factor behind pressure change?

We have Waters PDA Prep HPLC in our lab. Suddenly I am facing problem with fluctuation of pressure. What are the factors that can influence the pressure? How to solve this problem?

23 December 2012 2,979 16 View

What is the purpose of use acid/base like acetic acid, formic acid, NH4OH or silver nitrate in TLC or column chromatography using Silica gel?

How acid/base can give better separation of spots? Is it called derivatization? How do they work? Can anyone suggest a book that has a clear concept about it?

03 November 2012 5,932 2 View

In which situation/case would we need to adjust the pH of the mobile phase in TLC? How do I know that I need to adjust the pH? How do I adjust it?

Sometimes I see people adjust the pH of the mobile phase in TLC. How does this help in separation?

28 October 2012 3,648 4 View

What are suitable TLC solvent systems for Methanol extraction?

I am trying to develop a solvent system for HPLC analysis of my extracts.

20 October 2012 8,104 22 View

Solvent system for Thin Layer Chromatography of non polar extracts?

Can anyone suggest a suitable solvent system in TLC for n-hexane extracts to get better fractions for HPLC analyses?

13 July 2012 6,828 40 View