A.K.M. Moyeenul Huq

12 Questions 33 Answers 0 Followers

Questions related from A.K.M. Moyeenul Huq

How can a mixture of crystals obtained from plant extract can be separated?

I am isolating compounds from plant. i have got 2 types of crystals,one is needle like and another cubic in shape in a vial. now from here how can i separate them? any technique??

03 April 2015 5,603 6 View

Is a cDNA synthesis kit for realtime PCR compatible with conventional end point RT PCR?

I have been synthesizing cDNA using QuantiTech Rev Transcrp (Qiagen) kit to see the expression of TFF1 gene. In the label of the kit box it says that this kit synthesize cDNA which is specific for...

29 December 2014 2,491 8 View

Can anyone assist with identifying Plasmids from Glycerol stock?

I have three Plasmids in glycerol stock. I labelled them and kept in -80 freezer for 6-7 months. Now when I wanted to take them out from the freezer I found the the labels disappeared. Now is...

18 August 2014 2,288 12 View

How to prepare a primer solution?

I have primers from Integrated DNA Technologies in lyophilized form. The amount of Oligo is 25.5 nMoles = 0.16 mg. Now how should I dissolve it? Which solvent should I use? And how much of it?

25 March 2014 6,620 11 View

Can anyone advise on a cell culture problem?

I am culturing MCF-7 cancer cell line in RPMI with phenol red and without phenol red for my experiment. 10% FBS is used. The passage is 42. For the last couple of passages I see that the cells are...

18 January 2014 4,657 10 View

Can anyone help with making RNase free?

If I don't have DEPC/DEPC treated water, what alternative can I use to make my working pipette, tubes, tips RNase free ? And how?

18 December 2013 1,869 8 View

Can someone advise on plasmid purity and quality?

I am going to perform transfection of plasmids. I have got them from Addgene. 1st time I extracted plasmid using a sample mini prep kit from a company the quality was good. But next time I used...

06 July 2013 4,089 2 View

How to calculate the volume of medium containing the cell (MCF7 cell line) needed to seed 3000 cell/well in 96 well plate for MTT assay?

I want to perform an MTT assay for a cell proliferation study. After culturing the cells, when we need to seed the cells in a 96 well plate then how would I calculate the volume of cell containing...

22 March 2013 6,024 5 View

For Water extract what mobile phase can I use for HPLC? How should I prepare my sample?

I want to run HPLC for my Water extract. For my plant no paper found to chose a solvent system for HPLC.

24 December 2012 9,517 8 View

Problem with HPLC: its pressure is not getting stable. How can I solve this problem? What are the factor behind pressure change?

We have Waters PDA Prep HPLC in our lab. Suddenly I am facing problem with fluctuation of pressure. What are the factors that can influence the pressure? How to solve this problem?

23 December 2012 2,881 16 View

What is the purpose of use acid/base like acetic acid, formic acid, NH4OH or silver nitrate in TLC or column chromatography using Silica gel?

How acid/base can give better separation of spots? Is it called derivatization? How do they work? Can anyone suggest a book that has a clear concept about it?

03 November 2012 5,881 2 View

In which situation/case would we need to adjust the pH of the mobile phase in TLC? How do I know that I need to adjust the pH? How do I adjust it?

Sometimes I see people adjust the pH of the mobile phase in TLC. How does this help in separation?

28 October 2012 3,548 4 View