If an article is unknowingly published in a fake journal, is it considered as published or can the article be sent to some other journal to publish?

How do we handle this menace of fake journals?

14 July 2020 4,835 7 View

Do we have a relationship between emissivity of material and temperature?

Not the radiative heat transfer (Q=sigma*A*e(Ta^4 - Tb^4)) equation. In other words, can we directly conclude, for a given emissivity this will be your temperature? Like the Infra Red...

05 December 2019 6,670 1 View

Extinction cross-section for Nano-particle on a film - Lumerical/COMSOL?

Simulating (Lumerical solutions) a gold nanoparticle (NP) on a gold film structure. For NP in free space, we confirm the simulations results with Mie theory. How about in case of NP on a film...

25 July 2018 2,597 14 View

On what conditions dopants replace the host elements?

I doped Sn-Sb alloy with Ti,Cu and Ce elements individually (@ 25 wt % as replacement to Sn). From EDX calculation, the elemental compositions are Sn1.38Sb3.60, Sn0.85Ti0.37Sb3.86,...

30 January 2018 9,990 2 View

What is number of motor neurons in the median Nerve which innervates thenar muscles?

Hi, I am working on signal processing related to median Nerve and I need to know the number of motor neurons present in the median nerve. Thank you

11 January 2018 594 1 View

What is the zeta potential value of deionised or distilled water?

I am getting zeta potential value of about -30 mV for distilled water obtained from a commercial supplier. What it means? What should be the zeta potential value of distilled water? How can I...

10 October 2017 2,791 19 View

What is the reason for new peak of LLTO in Raman spectra?

18 August 2016 7,596 3 View

Zeta potential for amine functionalized Nanoparticles?

What will be the sign of zeta potential for amine functionalized nanoparticles? The amines are neutral (not protonated). Due to the electron rich character of amine functional group shall I expect...

30 July 2016 9,866 4 View

How to convert reference electrode potentials?

In cyclic voltammetry reports, when the potential values are given with respect to Li metal reference electrode, how can we convert this potential to other reference electrode such as SCE or Ag/AgCl

03 June 2016 2,562 3 View

Is the zeta potential value valid for amino acid functionalized nanoparticles?

I have modified my carbon nanoparticle surface with amino acids. Now I would have both amine and acid functionalities on the surface of my nanoparticle. The measured zeta potential value is about...

31 December 2015 6,592 10 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View