Can somebody recommend me an oxytocin producing neuronal cell line?

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How do you confirm the antibacterial activity is due to phytoconstituents and not due to solvent residues?

25 February 2024 9,631 1 View

Suggest any good article on Gel Propellants used in aerospace applications. Also are gel propellants currently in use?

gel propellant, rheology, aerospace

23 January 2024 9,151 1 View

Is it possible to make transesterification production of biodiesel continuous? or at least the secondary washing process with the aid of RO membrane?

The transesterification reaction produce Crude Biodiesel. This needs to be refined to meet the standard Biodiesel. This article investigates the ability of... Making the secondary washing...

13 March 2023 7,404 2 View

What Refractive Index to use for barley flour particle size analysis?

Hi, I am looking to use Mastersizer 3000E for particle size analysis of barley flour. I was wondering what refractive index to use for this. I know most papers suggest RI of 1.57 for wheat, would...

08 January 2022 6,189 1 View

If an article is unknowingly published in a fake journal, is it considered as published or can the article be sent to some other journal to publish?

How do we handle this menace of fake journals?

14 July 2020 5,037 7 View

How to calcualate area under curve (AUC) in SPSS?

How to get the area under the curve (AUC) when enrollment in health insurance (yes/no) is dependent and socio-demographic characteristics are independent variables? What are the applications of AUC?

24 May 2020 5,186 4 View

On what factors does coke moisture in a coke plant depend on, considering wet quenching is being practiced ?

The lesser the moisture in coke, the lesser the Coke rate in Blast furnaces and hence lesser fuel cost. In order to decrease the coke moisture, various parameters need to be controlled. A few Coke...

01 January 2020 5,907 1 View

Do we have a relationship between emissivity of material and temperature?

Not the radiative heat transfer (Q=sigma*A*e(Ta^4 - Tb^4)) equation. In other words, can we directly conclude, for a given emissivity this will be your temperature? Like the Infra Red...

05 December 2019 6,830 1 View

What are the possible technical reasons for the corruption of .AVI file which is copied from a digital video recorder to a pen drive?

14 March 2019 6,802 0 View

Where can I get CCT curves for austenitic stainless steels?

Is it similar to that of eutectoid steels?

18 September 2018 9,586 1 View

An animal in not a rock (e.g., Koch 2013), it has an internal constitution that can be modified?

Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...

07 August 2024 648 0 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

Are the GABAergic neurons electrophysiologically equal in all brain areas?

Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?

04 August 2024 4,090 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View