Ramanan Devaraj

5 Questions 5 Answers 0 Followers

Questions related from Ramanan Devaraj

Can somebody recommend me an oxytocin producing neuronal cell line?

There is a commercial cell available for POMC secreting cell line - mHypo-POMC/GFP. Similarly I would like to use a cell line for Oxytocin. Thanks in advance Ramanan Devaraj

26 May 2017 6,439 2 View

Which Alkaline Phosphatase (ALP) kit is better/suitable for mES cells?

Q1. I am planning to use an ALP kit to detect the mES cells pluripotent/ differentiation status. Which would be the better (ALP) kit for this purpose? Q2. I generally passage mES cells every 2-3...

17 August 2016 3,022 2 View

Does anybody knows telomerase mutations (in) related to Werner Syndrome?

Telomerase mutations have been reported with several human genetic diseases such as DKC, IPF etc., I would like to know whether any telomerase (either in TERT, TERC or DKC1) mutations have been...

20 April 2015 2,498 5 View

How to avoid cell loss during Immunofluorescence staining procedure for nuclear proteins?

Hi I am trying to make slides for IF imaging of DNA damage response proteins in HT1080 cells. When I observed the cells, I lost ~60%-70% of cells… only few cells are stained in cover slip. Rest of...

13 October 2014 173 6 View

DTT or BME - which one is better for RNA isolation from 293T cells?

In a Qiagen RNA isolation kit either DTT or BME has to be added to the RLT buffer.

27 March 2014 5,089 4 View