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Questions related from Ramanan Devaraj
I am going to check oxytocin receptor, Cn43 and some other genes expression in mice uterus during labor (including my gene of interest). Thinking to use the RPA method to show a set of highly...
24 April 2020 4,142 0 View
There is a commercial cell available for POMC secreting cell line - mHypo-POMC/GFP. Similarly I would like to use a cell line for Oxytocin. Thanks in advance Ramanan Devaraj
26 May 2017 6,497 2 View
By In vitro differentiation methods, ES cells can be differentiated into neuronal cells. But I am looking for an in vitro method to establish oxytocin producing neurons (specifically) from neural...
10 February 2017 7,537 3 View
Q1. I am planning to use an ALP kit to detect the mES cells pluripotent/ differentiation status. Which would be the better (ALP) kit for this purpose? Q2. I generally passage mES cells every 2-3...
17 August 2016 3,062 2 View
Telomerase mutations have been reported with several human genetic diseases such as DKC, IPF etc., I would like to know whether any telomerase (either in TERT, TERC or DKC1) mutations have been...
20 April 2015 2,535 5 View
Hi I am trying to make slides for IF imaging of DNA damage response proteins in HT1080 cells. When I observed the cells, I lost ~60%-70% of cells… only few cells are stained in cover slip. Rest of...
13 October 2014 214 6 View
In a Qiagen RNA isolation kit either DTT or BME has to be added to the RLT buffer.
27 March 2014 5,141 4 View
