Can somebody provide me equivalent circuit and differential difference equation of gradient transmission line?

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What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Crosslinking is one of the ways of enzyme immobilization on different surfaces, in a similar way can a chemical reagent be coated/fixed?

Crosslinking on surfaces makes the enzyme reusable multiple times, I wish to know whether this is possible for a chemical reagent. Once fixed or coated on a surface, can the reagent be used...

29 January 2024 7,177 1 View

In hfss it does not showing far field setup in result menu and I already set up radiation values and analysed all but it does not shows option?

I'm trying to calculating far fieds after calculation of s-paramenters for my anteena design but it doesn't showing the far filed set up option in results menu even I gave radiation values HFSS...

02 January 2024 5,145 0 View

Nyquist plots for my solid-state electrolyte do not seem continious.. any insights??

Dear Researchers, Despite careful data collection and analysis, the plot appears to be broken at several points (image attached). These breaks seem to deviate from the expected pattern, and I'm...

28 November 2023 5,631 3 View

How polaron hopping is linked with MWS/Interfacial polarization in ferrite system?

Ferrites, hopping mechanism

22 September 2023 1,411 1 View

What is the stability of single guide RNAs at room temperature?

Hello fellow researchers!! This question is based on a very unfortunate dry ice shipment incident. Recently I booked a dry ice shipment for delivering Synthego's chemically modified gRNAs...

22 June 2023 974 0 View

How to design PSA Or VPSA system for biogas upgradation?

Want to upgrade biogas using vaccum pressure swing adsorption system. Looking for mathematical equations and parameters governing the design.

31 May 2023 2,592 2 View

Pretty new to maps here. Png to shapefile, Steps?

Greetings everyone. I have a png with highlighted areas and I need to convert it into a shapefile with all the pipelines as polygon features. Any help is appreciated.

05 March 2023 5,681 6 View

Crystal voids in coordination polymer?

How to calculate void in my coordination polymer through olex2 or any other software?

07 December 2022 7,536 1 View

Anyone knows any contact from NIBSC.org. "Genetic Reference Panel, for the quantitation of BCR-ABL translocation by RQ-PCR (1st I.S.)"?

Can anyone in the research gate community share contact details of personnel who works in NIBSC.org please. I need some details regarding there "Genetic Reference Panel, for the quantitation of...

04 November 2022 9,177 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View

What are some signs that an ECM is overfitting the Nyquist plot?

What can you look for to know that the circuit model being used is overfitting the data. How do you know when overfitting is occurring?

31 July 2024 7,425 0 View

Why running a restart analysis in Abaqus in Ubuntu OS gives an error as attached when running the same job in Windows doesn't give any error and runs?

I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...

29 July 2024 9,572 3 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View