Can I use a single polyclonal antibody batch split in two for a sandwich assay?

I have been using kits with paired monoclonal antibodies, what I wish to do is purchase polyclonal antibodies to a particular antigen and split in two, immobilise one half and fluoro label the...

31 December 2018 5,141 7 View

When measuring Zeta Potential in ethanol, is it necessary to add salt?

Hi, I'm new to this type of measurement, from what I've read, the addition of salt is vital in aqueous measurements of Z. However I have been measuring a silica micro-sphere suspension in pure...

06 July 2018 6,214 6 View

Can polyelectrolyte bi-layers be formed from organic solvent?

I have Raman functionalised Ag microspheres and wish to coat in polyelectrolyes, however when I transfer them to aqueous solvents for this it becomes far more difficult to wash the sample and I...

03 April 2018 3,942 0 View

Can Silica surface hydroxyl groups be used with cyanylating agent to immobilise protein?

In all literature I read the cyanylating agent 1-cyano-4-dimethylaminopryidinium tetrafluoroborate (CDAP) is used for cross-linking to sugars, in these we obviously have our hydroxyl oxygen...

02 March 2018 643 2 View

What alternatives are there to using Piranha solution for cleaning silica before silanization?

I have SERS microsphere sensors that have been silica coated, I have been coating them in APTES for further protein crosslinking. In order to get a good and even coating of APTES a lot of papers...

02 March 2018 4,659 5 View

Can low concentrations in SERS have a larger intensity?

I have been synthesising Ag nanoparticle films on Silica beads, I wished to show the limit of detection by making a concentration serial dilution of Rhodamine 6 G, however, there was strange...

02 March 2018 7,893 3 View

How to remove aggregates from Microsphere preparations?

09 October 2016 1,676 3 View

Can DLS or a Zeta Sizer be used to get particle concentration?

We have magnetite spheres that are polystyrene coated and I wish to get analysis that tells me the concentration before and after magnetic recovery. Can the above mentioned techniques work?

01 February 2016 323 6 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View