Can I use a single polyclonal antibody batch split in two for a sandwich assay?

I have been using kits with paired monoclonal antibodies, what I wish to do is purchase polyclonal antibodies to a particular antigen and split in two, immobilise one half and fluoro label the...

31 December 2018 5,141 7 View

When measuring Zeta Potential in ethanol, is it necessary to add salt?

Hi, I'm new to this type of measurement, from what I've read, the addition of salt is vital in aqueous measurements of Z. However I have been measuring a silica micro-sphere suspension in pure...

06 July 2018 6,214 6 View

Can Silica surface hydroxyl groups be used with cyanylating agent to immobilise protein?

In all literature I read the cyanylating agent 1-cyano-4-dimethylaminopryidinium tetrafluoroborate (CDAP) is used for cross-linking to sugars, in these we obviously have our hydroxyl oxygen...

02 March 2018 643 2 View

What alternatives are there to using Piranha solution for cleaning silica before silanization?

I have SERS microsphere sensors that have been silica coated, I have been coating them in APTES for further protein crosslinking. In order to get a good and even coating of APTES a lot of papers...

02 March 2018 4,659 5 View

Can low concentrations in SERS have a larger intensity?

I have been synthesising Ag nanoparticle films on Silica beads, I wished to show the limit of detection by making a concentration serial dilution of Rhodamine 6 G, however, there was strange...

02 March 2018 7,893 3 View

How to remove aggregates from Microsphere preparations?

09 October 2016 1,676 3 View

Any suggestions on antibody pairs for a simple sandwich immunoassay?

I'm developing a dual mode detection immunoassay, based on antibody conjugated microspheres for the first method and fluorescent tagged antibodies for the second. What I'm looking for is any...

08 September 2016 9,976 3 View

Can DLS or a Zeta Sizer be used to get particle concentration?

We have magnetite spheres that are polystyrene coated and I wish to get analysis that tells me the concentration before and after magnetic recovery. Can the above mentioned techniques work?

01 February 2016 323 6 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View