Daragh Rice

9 Questions 12 Answers 0 Followers

Questions related from Daragh Rice

Can I use a single polyclonal antibody batch split in two for a sandwich assay?

I have been using kits with paired monoclonal antibodies, what I wish to do is purchase polyclonal antibodies to a particular antigen and split in two, immobilise one half and fluoro label the...

01 January 2019 5,203 7 View

When measuring Zeta Potential in ethanol, is it necessary to add salt?

Hi, I'm new to this type of measurement, from what I've read, the addition of salt is vital in aqueous measurements of Z. However I have been measuring a silica micro-sphere suspension in pure...

07 July 2018 6,310 6 View

Can polyelectrolyte bi-layers be formed from organic solvent?

I have Raman functionalised Ag microspheres and wish to coat in polyelectrolyes, however when I transfer them to aqueous solvents for this it becomes far more difficult to wash the sample and I...

04 April 2018 4,005 0 View

Can Silica surface hydroxyl groups be used with cyanylating agent to immobilise protein?

In all literature I read the cyanylating agent 1-cyano-4-dimethylaminopryidinium tetrafluoroborate (CDAP) is used for cross-linking to sugars, in these we obviously have our hydroxyl oxygen...

03 March 2018 716 2 View

What alternatives are there to using Piranha solution for cleaning silica before silanization?

I have SERS microsphere sensors that have been silica coated, I have been coating them in APTES for further protein crosslinking. In order to get a good and even coating of APTES a lot of papers...

03 March 2018 4,734 5 View

Can low concentrations in SERS have a larger intensity?

I have been synthesising Ag nanoparticle films on Silica beads, I wished to show the limit of detection by making a concentration serial dilution of Rhodamine 6 G, however, there was strange...

03 March 2018 7,960 3 View

How to remove aggregates from Microsphere preparations?

I've got microspheres at approx 1.5µm diameter, these undergo several coating steps, one step is to coat with silver and another with polyelectrolytes. These steps cause aggregation and I'm left...

10 October 2016 1,732 3 View

Any suggestions on antibody pairs for a simple sandwich immunoassay?

I'm developing a dual mode detection immunoassay, based on antibody conjugated microspheres for the first method and fluorescent tagged antibodies for the second. What I'm looking for is any...

09 September 2016 10,039 3 View

Can DLS or a Zeta Sizer be used to get particle concentration?

We have magnetite spheres that are polystyrene coated and I wish to get analysis that tells me the concentration before and after magnetic recovery. Can the above mentioned techniques work?

02 February 2016 381 6 View