HI
is it possible that a recombinant protein/ protein from other source (like-bacteria/plant/ yeast) can go inside the single cell ( animal cell culture) and affect the metabolic pathway or rescue the effect of the particular knockout gene?
Thanks
It is very rare that proteins enter the cell (except for some antibodies and other proteins. see the links). Proteins and other macromolecules enter the cell mostly through pinocytosis and phagocytosis, which leads to their degradation. Some small peptides are known to enter the cells. Viral proteins as a whole virus particle enter the cell by through escape after phagocytosis or fusion of their membrane. The other ways of protein transport between cells are cell-cell junctions and exosomes. I hope this information is helpful to you. More information can be found in the following links.
https://www.ncbi.nlm.nih.gov/pubmed/22840771
http://www.jbc.org/content/281/34/24803.full
http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0854.2010.01041.x/full
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038006/
It is very rare that proteins enter the cell (except for some antibodies and other proteins. see the links). Proteins and other macromolecules enter the cell mostly through pinocytosis and phagocytosis, which leads to their degradation. Some small peptides are known to enter the cells. Viral proteins as a whole virus particle enter the cell by through escape after phagocytosis or fusion of their membrane. The other ways of protein transport between cells are cell-cell junctions and exosomes. I hope this information is helpful to you. More information can be found in the following links.
https://www.ncbi.nlm.nih.gov/pubmed/22840771
http://www.jbc.org/content/281/34/24803.full
http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0854.2010.01041.x/full
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3038006/
Thank you for the answer. The links you provided will for sure clarify my doubts and will be helpful.
Hi there,
Only some bacterial toxins (like diphteria toxin for instance) are able to do so in order to kill their target cell. Attempts to use their translocation properties for therapeutics delivery are currently examined... See the following:
https://www.ncbi.nlm.nih.gov/pubmed/26103531
The short answer is Yes. It depends on a number of factors- including the protein sequence, size, and charge. Therefore, if more information about the recombinant protein is available, it would possible to predict the chances of it getting into a cell.
Dear Dominique Liger and Divaker Choubey,
Thanks for the answers.
Dominique Liger, the link you provided will help to understand the issue.
I would just not like to say no to your question. It will depend upon the nature of the protein, its site of attachment on the cell membrane etc. To give you a classical example, is that of the recombinant Diphtheria toxin which is prepared to bring about apoptosis of certain neoplasm cells. This recombinant protein attaches on certain chemo receptors of these abnormal cells and then gets transported inside the cells to bring about the process of apoptosis.
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