I am facing problem in dissolving pNPP in 1X diethanolamine. Could any body help me to figure it out?

Hello, We bought pNPP tablets and 5X buffer ( diethanolamine) from Pierce in our laboratory. I have to make 300mM stock solution, (as i can't go to lower concentration for making stock) and it is...

07 August 2015 6,490 1 View

Why MgCl2 is required in all phosphatase assays?

Why MgCl2 is required in all phosphatase assays? Can anybody tell what it does in assay reactions? Thanks

03 April 2015 3,138 2 View

Can any one provide UV-vis Spectra and other related infromation about hydroquinone .

I am trying to make hydroquinone from benzoquinone enzymatically.

11 December 2012 1,906 5 View

Different intensity of blue color, during dna preparation after adding p2 buffer.

DNA preparation.

08 September 2012 8,621 5 View

A lot of colonies from PCR product

I was doing mutation using PCR, the amount of plasmid that I took was greater than amount given in protocol for digestion of the parental DNA, incubated for more than 4 hours at 37C, then...

07 August 2012 3,400 5 View

Can recombinant protein go inside the cell in animal cell culture?

01 January 1970 1,379 9 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

Why negative controls also showing the specific bands of promotor and other regions (GUS, GFP etc.)?

Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...

06 January 2021 8,465 99 View