Different intensity of blue color, during dna preparation after adding p2 buffer.

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Chelladurai Rathnasingh

have you used qiagen? if so, they recently introduced LysBlue. when P2 added, make sure that the P2 buffer mixed well without any white patches. same way, when P3 added, the complete decoloriztion of the lysate making sure the mixing of P3

Mário José Ferreira

That can happen simply because your samples have different amounts of cells.

Blue color is proportional to the level of cell lysis, so if one of your samples has less cells to begin with you'll necessary have a lighter blue color.

GAËL NICOLAS

I am using preps from this company but i don't like the LysBlue. So i did not put it into P1 bottle.

Vinita Yadav

Thanks Mario for your information on blue color appeared during cells lysis.

Happy to help you Vinita.