I am facing problem in dissolving pNPP in 1X diethanolamine. Could any body help me to figure it out?

Hello, We bought pNPP tablets and 5X buffer ( diethanolamine) from Pierce in our laboratory. I have to make 300mM stock solution, (as i can't go to lower concentration for making stock) and it is...

07 August 2015 6,490 1 View

Why MgCl2 is required in all phosphatase assays?

Why MgCl2 is required in all phosphatase assays? Can anybody tell what it does in assay reactions? Thanks

03 April 2015 3,138 2 View

Can any one provide UV-vis Spectra and other related infromation about hydroquinone .

I am trying to make hydroquinone from benzoquinone enzymatically.

11 December 2012 1,906 5 View

A lot of colonies from PCR product

I was doing mutation using PCR, the amount of plasmid that I took was greater than amount given in protocol for digestion of the parental DNA, incubated for more than 4 hours at 37C, then...

07 August 2012 3,400 5 View

Hello friends,

The question is for the people working on fungal proteins. Please tell me on which protein or enzyme from fungus you are working. Thanks vinita

31 December 2011 4,619 5 View

Can recombinant protein go inside the cell in animal cell culture?

01 January 1970 1,379 9 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Any stable dye to color acrylamide hydrogel beads?

Hi! We are generating hydrogel beads(50-60um) using microfluidics as single-cell beads. To simply all the experiments (especially the centrifuge), I am looking for a dye. The dye should be stable...

14 January 2021 4,383 8 View

How to phosphorylate specific residues on pymol?

Hi everyone, I am using the pyTMs plug in on Pymol to phosphorylate a particular threonine in my protein. I am having difficulty in selecting only one or two residues - it seems I can only...

05 January 2021 230 3 View