Can ELISA be used instead of IHC to detect neurogenesis?

To quantify neurogenesis in the dentate gyrus, instead of doublecortin IHC, can I use doubecortin ELISA by using hippocampal tissue homogenate?. I can not find any reference articles for detection...

08 September 2017 4,985 3 View

Do my experiments get affected by 4% PFA?

11 December 2016 9,508 2 View

Are there any articles published about cerebral dominance in rodents (rats) and histological changes based on the cerebral dominance?

Are there any articles published about cerebral dominance in rodents (rats) and histological changes based on the cerebral dominance?

01 February 2015 3,570 1 View

Do you know a suitable ELISA protocol for TGF Beta 1 analysis?

I am looking for a ELISA protocol to analyse TGF-Beta 1 using rat hippocampal homogenate. Can anyone suggest, which species antibody should I use for it to get good result (I will be using rat...

31 December 2014 2,493 5 View

What is 'AMFIX'?

06 July 2014 2,062 3 View

Are there any immunohistochemical methods to look for dendritic arborization of hippocampal neurons in rats?

Currently I am working in the area of adult neurogenesis. I am using Golgi-Cox and Scholl's method to analyse dendritic arborization (though its not specific for newborn neurons). Can I use SMI-32...

02 March 2014 7,573 3 View

In order to create spinal cord injury model, how to identify T10 vertebra in a rat?

How to identify T10 vertebra in a rat, in order to create spinal cord injury? Are there any anatomical landmark/s to find out T10? I have searched for details in few literature (review and...

07 August 2013 5,679 10 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View