Can ELISA be used instead of IHC to detect neurogenesis?

To quantify neurogenesis in the dentate gyrus, instead of doublecortin IHC, can I use doubecortin ELISA by using hippocampal tissue homogenate?. I can not find any reference articles for detection...

08 September 2017 4,985 3 View

Do my experiments get affected by 4% PFA?

11 December 2016 9,508 2 View

Do you know a suitable ELISA protocol for TGF Beta 1 analysis?

I am looking for a ELISA protocol to analyse TGF-Beta 1 using rat hippocampal homogenate. Can anyone suggest, which species antibody should I use for it to get good result (I will be using rat...

31 December 2014 2,493 5 View

What is 'AMFIX'?

06 July 2014 2,062 3 View

Are there any immunohistochemical methods to look for dendritic arborization of hippocampal neurons in rats?

Currently I am working in the area of adult neurogenesis. I am using Golgi-Cox and Scholl's method to analyse dendritic arborization (though its not specific for newborn neurons). Can I use SMI-32...

02 March 2014 7,573 3 View

Can radial glia be visualized?

Can they be visualized by immunohistochemistry of brain sections, especially the subventricular zone of wistar rat pup during the postnatal day 15 or 30?

31 December 2013 5,472 4 View

In order to create spinal cord injury model, how to identify T10 vertebra in a rat?

How to identify T10 vertebra in a rat, in order to create spinal cord injury? Are there any anatomical landmark/s to find out T10? I have searched for details in few literature (review and...

07 August 2013 5,679 10 View

How can we calculate the sample size for rodents for a particular period of time?

How can we calculate the sample size for rodents for a study for one year? Are there any specific protocols to be followed for designing rodent sampling? How long should be the sampling interval...

01 March 2021 1,056 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View