8 Questions 4 Answers 0 Followers
Questions related from Dr. Rajesh Thangarajan
To quantify neurogenesis in the dentate gyrus, instead of doublecortin IHC, can I use doubecortin ELISA by using hippocampal tissue homogenate?. I can not find any reference articles for detection...
09 September 2017 5,049 3 View
I have gone through different protocols to prepare 4% PFA for perfusion, now I am confused, which of these PFA preparation method I should follow: a) 4% PFA in 0.1M PBS, pH 7.4 or b) 4% PFA in...
12 December 2016 9,574 2 View
Are there any articles published about cerebral dominance in rodents (rats) and histological changes based on the cerebral dominance?
02 February 2015 3,658 1 View
I am looking for a ELISA protocol to analyse TGF-Beta 1 using rat hippocampal homogenate. Can anyone suggest, which species antibody should I use for it to get good result (I will be using rat...
01 January 2015 2,565 5 View
I am interested to do neuronal tracing by Golgi- Cox modified method. In order to perform this method - brain tissue sections has to be decolorized in 15% amfix (ref: Theory and practice of...
07 July 2014 2,121 3 View
Currently I am working in the area of adult neurogenesis. I am using Golgi-Cox and Scholl's method to analyse dendritic arborization (though its not specific for newborn neurons). Can I use SMI-32...
03 March 2014 7,640 3 View
Can they be visualized by immunohistochemistry of brain sections, especially the subventricular zone of wistar rat pup during the postnatal day 15 or 30?
01 January 2014 5,543 4 View
How to identify T10 vertebra in a rat, in order to create spinal cord injury? Are there any anatomical landmark/s to find out T10? I have searched for details in few literature (review and...
08 August 2013 5,801 10 View
