No title

Dear colleagues, In my experience, some evaluations received in the peer review process of journals, often include criticisms based on the QUANTITY of examples you can claim you have in a...

06 November 2019 8,055 4 View

A quick immunology question: Does this immunology graph seem correct?

Hi!! I have created a graph about the immune response in healthy and AD skin for S. aureus. Could I please ask if it should be a table or a figure? Does the pathway look correct? Shall I make it...

20 October 2019 1,706 3 View

Is there a "gold standard" for amino acid derivatization for LC/MS analysis?

We've recently started using a single quad LC/MS system (Shimadzu LC/MS-2020, for what it's worth) for mouse plasma AA analysis (the 20 common EAA and NEAA). Pre-column derivatization seems to be...

01 January 2019 1,398 5 View

50-50 dilution in elisa is better than no dilution?

Hi, I have been doing HBD-3 Elisa with highest sample concentration 50-50 dilution. I heard that 50-50 is better than adding 100uL of your sample, because you take into account the diluent as...

15 September 2018 3,118 3 View

Transformation efficiency in Corynebacterium glutamicum using GG ligation mix?

What steps can I take to increase the transformation efficiency of electroporated Cg? When transforming with a plasmid from E. coli, the efficiency is very high and I have ample colonies to choose...

25 July 2018 5,889 1 View

Has anyone identified the compound 1,2-Benzenedicarboxylic acid, mono (2-ethylhexyl) ester in plant material?

I have found 3 citations where it has been extracted from plant material but I would need to know if someone else has found it since in my samples it is in a percentage higher than 20%

14 May 2018 480 3 View

De-salting methods without losing any proteins?

Hi, I am looking for methods of removing the salt from a solution, without using buffer exchange dialysis, or spinning down using membranes, so that no proteins are lost, no matter how small they...

28 March 2018 1,356 3 View

WB to detect Gft2h4, I don't have the band, but I have a perfect band on the control (H3), 4 different Ab tried and dot blot to, any suggestion about?

I'm doing WB to detect Gtf2h4 in mice's thymus and spleen. I used 4 different Ab from different companies and no one seems to work, but the positive control (histone 3) was perfect. I tried with...

19 October 2017 764 2 View

Protocol for bacteria competition assays?

Hi, I would like to perform competition assays for two staphylococcal strains with and without reagents in order to see which one grows better. Do you have any protocol for this? I was thinking of...

25 October 2016 5,616 2 View

How do I dissolve D-Aspartic Acid and Ala-Phe for MICs?

19 October 2016 4,587 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

Mixture neutralization after Boc deprotection ?

I am performing a Boc-deprotection with HCl in organic solvent (dioxane or Ethylacetate) but would like to isolate the free amine. Several problems: - Neutralizing with inorganic base as NEt3...

18 February 2021 3,366 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Onset of Photo Oxidation in Polymers?

Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...

15 February 2021 7,703 4 View

What could be the possible causes of DNA degradation after extraction from 4% gel?

Hi everyone, During small RNA library preparation, I run the samples in 4% agerose gel to select the band corresponding to my target. I established beforehand the protocol and it works fine....

02 February 2021 5,008 2 View