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Dear colleagues, In my experience, some evaluations received in the peer review process of journals, often include criticisms based on the QUANTITY of examples you can claim you have in a...

06 November 2019 8,055 4 View

A quick immunology question: Does this immunology graph seem correct?

Hi!! I have created a graph about the immune response in healthy and AD skin for S. aureus. Could I please ask if it should be a table or a figure? Does the pathway look correct? Shall I make it...

20 October 2019 1,706 3 View

Is there a "gold standard" for amino acid derivatization for LC/MS analysis?

We've recently started using a single quad LC/MS system (Shimadzu LC/MS-2020, for what it's worth) for mouse plasma AA analysis (the 20 common EAA and NEAA). Pre-column derivatization seems to be...

01 January 2019 1,398 5 View

50-50 dilution in elisa is better than no dilution?

Hi, I have been doing HBD-3 Elisa with highest sample concentration 50-50 dilution. I heard that 50-50 is better than adding 100uL of your sample, because you take into account the diluent as...

15 September 2018 3,118 3 View

Transformation efficiency in Corynebacterium glutamicum using GG ligation mix?

What steps can I take to increase the transformation efficiency of electroporated Cg? When transforming with a plasmid from E. coli, the efficiency is very high and I have ample colonies to choose...

25 July 2018 5,889 1 View

Has anyone identified the compound 1,2-Benzenedicarboxylic acid, mono (2-ethylhexyl) ester in plant material?

I have found 3 citations where it has been extracted from plant material but I would need to know if someone else has found it since in my samples it is in a percentage higher than 20%

14 May 2018 480 3 View

De-salting methods without losing any proteins?

Hi, I am looking for methods of removing the salt from a solution, without using buffer exchange dialysis, or spinning down using membranes, so that no proteins are lost, no matter how small they...

28 March 2018 1,356 3 View

WB to detect Gft2h4, I don't have the band, but I have a perfect band on the control (H3), 4 different Ab tried and dot blot to, any suggestion about?

I'm doing WB to detect Gtf2h4 in mice's thymus and spleen. I used 4 different Ab from different companies and no one seems to work, but the positive control (histone 3) was perfect. I tried with...

19 October 2017 764 2 View

Protocol for bacteria competition assays?

Hi, I would like to perform competition assays for two staphylococcal strains with and without reagents in order to see which one grows better. Do you have any protocol for this? I was thinking of...

25 October 2016 5,616 2 View

How do I dissolve D-Aspartic Acid and Ala-Phe for MICs?

19 October 2016 4,587 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View