I'm unfamiliar with ESC culture. Can they be grown into confluent monolayers? Do they form epithelial adherens junctions?
I'm having trouble sorting through the literature, as pubmedding those terms comes up with a ton of noise (like ESC being differentiated into something with adherens junctions).
I've heard of small micropatterned cultures like
http://www.nature.com/nmeth/journal/v11/n8/abs/nmeth.3016.html
But I'm wanting to know if a flat uniform epithelial layer of pluripotent cells is possible. If so, is there a size limit on it?