8 Questions 5 Answers 0 Followers
Questions related from Philip Spear
I'm having trouble finding head-to-head comparisons of lipofectamine vs nanoparticles like silica or gold for transfecting high levels of DNA into cells in culture. What's the best way to get...
08 January 2018 8,131 3 View
In terms of cell culture media and other consumables, what's the cheapest eukaryotic cell to culture? FBS is expensive, serum-free alternatives for cells that require FBS are even more expensive....
04 December 2017 6,868 3 View
I'm trying to grow a confluent layer of epithelial cells on a filter, then would like to disperse the layer off the membrane. I don't want individual cells to disperse, I want the cell-cell...
23 March 2017 1,826 1 View
I'm now needing to image cell cultures. I am thinking about transiently transfecting a fluorescent DNA marker, but I think it will be too sparsely labeled for my purposes. And throwing a dye on...
08 March 2017 2,570 17 View
I'm unfamiliar with ESC culture. Can they be grown into confluent monolayers? Do they form epithelial adherens junctions?I'm having trouble sorting through the literature, as pubmedding those...
09 December 2016 8,727 1 View
I'm hoping to get a single, confluent epithelial layer of hacat cells growing on top of a layer of matrigel. I can find people culturing hacat cells IN matrigel, but not a smooth layer of each.
19 April 2016 7,694 1 View
I have hundreds of LSM files from a zeiss microscope. Each one is a 3D confocal image time point. The software should automatically concatenate these into a single file, but it didn't this time. ...
05 February 2015 492 5 View
I'm growing MDCK cells on polyacrylimide gels. I am using sulfo-SANPAH to crosslink collagen to the PAAM. Link included to some pictures I took... on my phone through the eyepiece. There are...
20 November 2014 9,881 5 View
