Can neuron like cell lines be used to study neurodegeneration?

More Narendrakumar Ravi Varadharajulu's questions See All

Need a reference on pig residual analysis report and the parameters containing in it, for an oil and gas pipeline industry. Has anybody done it?

Pigging is basically a cleansing process followed in all pipeline industries after the product has been delivered. There are many types of pigs that are inserted inside the pipeline for this...

27 June 2024 1,221 1 View

HI, How to find phase response in CST?

I'm simulating a metamaterial unit cell. I want to plot Frequency Vs Phase Response. Also I want to extract permeability and permittivity. Kindly share the steps.

23 June 2024 3,446 1 View

Yeast Cell Glucose Uptake Assay ? Can yeast release more glucose into the reaction, when treated with test compound ?

Hi all, We performed Yeast-Cell-Glucose-Uptake assay to estimate the antidiabetic potential of the test compound. The Metformin treated yeast cells, resulted in taking up more glucse than normal...

02 April 2024 7,657 2 View

Suggest good geospatial data security papers ?

pursuing phd on geospatial data security.suggest good paper on robust invisible blind watermarking for copyright protection

12 March 2024 4,917 2 View

What is the difference between the single microgrid and multi microgrid? why we consider Multi microgrid in an area but not as a single microgrid ?

in a community area, there is an multi microgrid systems, why we call it as multi microgrid system but not treated as single microgrid system. and difference between single microgrid and multi...

03 March 2024 9,306 1 View

Wire bonding issue (Au/Ti/GaN). Any feasible solutions please?

I am facing an issue of wire bonding, trying to ball bond Au wire onto a metal stack/semiconductor (Au/Ti/GaN). Thickness of Au = 200 nm, Thickness of Ti = 20 nm. I observe that the metal...

07 January 2024 4,813 1 View

How to calculate the precision for a RT PCR assay?

I am studying precision for my assay/kit with the following parameters followed: Panel members to be tested: 1. Weak positive 2. Medium positive 3. Strong positive 4. Negative All these were...

11 December 2023 1,812 1 View

How to add my articles in research gate?

how can i add all my articles and journals in research gate including those published in research gate?

22 November 2023 5,926 3 View

Statins causes liver damage upon prolonged use. To control it, given natural with hepatoprotective activity. But results HDI! what might be the cause?

Atorvastatin used to treat Atherosclerosis, seems to causes hepatoxicity upon prolonged usage. So, to inhibit such problem we have introduced an natural substances which is reported for the...

09 November 2023 587 0 View

Why monocytes adhere while differentiating them as Dendritic cells?

I prepared PBMCs,s from human blood and differentiated them as immature dendritic cells in this process, monocytes adhere to the plates. What could be the reason for this phenotype?

09 November 2023 8,266 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Michelangelo Certo

One cell culture model that will surely play an important role in PD research and that already combines many of the aforementioned aspects consists of dopaminergic neurons derived from human induced pluripotent stem cells redirected from human fibroblasts. (Takahashi et al., 2006).

Embryonic midbrain neurons from embryonic day 14 (E14), which can be exposed to various neurodegenerative stimuli.

A commonly human cell line used in PD research is the HEK293 (human embryonic kidney 293) cell line.

Pavel Montes de Oca B

shsy-5y neuroblastoma cells have been reported in excitotoxicity assays