Does compensation need to be done on each treatment group to study the effect of treatments on the cell surface marker expression of a cell line?

I would like to investigate the effect of treatments on the cell surface expression of a cell line. Assuming that one treatment upregulates FITC-stained marker and the level of PE-stained marker...

07 August 2016 3,821 3 View

Could we define the cancer stem cell subpopulation within a cancer cell line solely based on its expression of aldehyde dehydrogenase (ALDH)?

Read about distinguishing cancer stem cells (CSCs) from its non-CSC counterpart based on stem cell surface markers, such as CD44, CD24, and CD133, is ambiguous and questionable. Is aldehyde...

05 June 2015 3,872 12 View

Does western blot documentation system capture chemiluminescence as good as X-ray film?

Does anyone have experience on both X-ray film development and western blot documentation system, who could comment on which method excels the other as a better way to detect and document the...

05 June 2015 9,303 5 View

Is inconsistency commonly seen in replicates of cell immunophenotyping?

I would like to immunophenotype cancer cell lines based on the expression of cell surface markers. I have done three individual experiments with cells of different passage number, which is at...

04 May 2015 6,008 3 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View