Yuan Han Teh

5 Questions 15 Answers 0 Followers

Questions related from Yuan Han Teh

Does compensation need to be done on each treatment group to study the effect of treatments on the cell surface marker expression of a cell line?

I would like to investigate the effect of treatments on the cell surface expression of a cell line. Assuming that one treatment upregulates FITC-stained marker and the level of PE-stained marker...

08 August 2016 3,863 3 View

Can laminin-coated culture vessels be reused for multiple cell passaging?

Do multiple times of cell dissociation inactivate the laminin activity? If the laminin-coated culture vessels can be reused several times, what is the limit to the number of times?

02 February 2016 405 3 View

Does western blot documentation system capture chemiluminescence as good as X-ray film?

Does anyone have experience on both X-ray film development and western blot documentation system, who could comment on which method excels the other as a better way to detect and document the...

06 June 2015 9,352 5 View

Could we define the cancer stem cell subpopulation within a cancer cell line solely based on its expression of aldehyde dehydrogenase (ALDH)?

Read about distinguishing cancer stem cells (CSCs) from its non-CSC counterpart based on stem cell surface markers, such as CD44, CD24, and CD133, is ambiguous and questionable. Is aldehyde...

06 June 2015 3,936 12 View

Is inconsistency commonly seen in replicates of cell immunophenotyping?

I would like to immunophenotype cancer cell lines based on the expression of cell surface markers. I have done three individual experiments with cells of different passage number, which is at...

05 May 2015 6,052 3 View