We extract the full eye from a mouse, and then fix it in 4% paraformaldehyde immediately after extraction. After punching a hole in the cornea, we fix it with 4% PFA in 20 degrees for 4 hours, and then we put the eye into a sucrose gradient 10% 20% and then 30% sucrose overnight. The tissues are put into OCT medium cryomold and sectioned onto superfrost slides. Will these tissues be acceptable for In Situ Hybridization? Do I need to refix before pretreating the tissues?

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