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Questions related from Matthew J Tarchick
Hello, I am looking at protein expression across mouse retina. We use littermate controls so many times our sample size is limited to 4-8 samples. To ensure protein does not degrade, we usually...
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I am having problems with DNA extraction of a tail sample. Here is the procedure I've been using. 300 uL of cell lysis solution, then I use a blue microtube pestle to break up the tail. I add 1.5...
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I am doing a RT-qPCR of mouse retinas. Each retina is very small. Can I use right and left retinas seperately, or would I need to use both a right and left retina in one mix to get enough sample?
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We extract the full eye from a mouse, and then fix it in 4% paraformaldehyde immediately after extraction. After punching a hole in the cornea, we fix it with 4% PFA in 20 degrees for 4 hours, and...
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