How many retinal samples are needed for PCR?

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Margaret Kenyon Applebee

Usually very little RNA is needed for RT-qPCR, but exactly how much you need depends on the target and specific assay, which usually you want to optimize. Through the optimization process, you will find out how many assays you can run with whatever the typical amount of RNA extracted from one retina is.

Saleh Alkarim

Hello

i agree with Margaret Kenyon Applebee and Aarti Kuver

Also , please see the article in attachment

Good luck

John Michael Powers

Hi,

I am currently working on a project that involves RNA and DNA isolation, but I'm not sure how efficient previous attempts have been. I was wondering if you would be willing to provide any insight into the recovery levels you obtained from retinas.