As above
Thank you very much
if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...
24 February 2021 357 2 View
The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.
21 February 2021 4,733 19 View
Hi Colleagues- What do you think about the following methodological issues: 1. Is it methodologically wrong to formulate quantitative research questionnaire based on inductive reasoning? 2. Is it...
21 February 2021 4,634 6 View
the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please
16 February 2021 8,801 3 View
Are there alternatives to the Devarda alloy when estimating nitrogen? devarda alloy is reducing the nitrate to ammonium
15 February 2021 8,838 3 View
i want to implement pseudo random number generator in matlab ...i request to every one if someone have code these PRNG share with me SplitMix Permuted Congruential Generator (PCG) Random...
14 February 2021 6,828 7 View
Up to what extent website personalization can help in creating urge to buy impulsively? The digital transformation is making most of the organizations to go online and start doing business through...
09 February 2021 4,430 6 View
Hello everybody My research involves an intervention where an instructor delivers a training course to students. My role as a researcher is to write down observations. Do you have any idea about...
03 February 2021 6,853 3 View
We want to conduct a verbal autopsy using WHO adult VA updated tools in ODK. The current version of WHO adult verbal autopsy tools is v1.5.3. I have successfully converted VA ODK-XLS v1.4 into XML...
03 February 2021 844 2 View
I am working with a large dataset that has many categorical variables with many classes in each variable. I want to encode the categorical variables without increasing the dimensionality of the...
31 January 2021 6,986 3 View
Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...
03 March 2021 4,275 4 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...
02 March 2021 1,408 1 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...
02 March 2021 9,157 1 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
