Hi there.
I want to extract separately cytoplasmic and nuclear RNA fraction from leukemic cell line by Trizol. Most of protocols that I saw suggests using a buffer with NP-40 or Triton X-100 for cell lysis. I have a little bit of NP-40, but large amount of Tween-20. May such detergent be suitable for such purpose? If yes, how I can adjust the concentration for extraction?
Thanks in adwance for help!