Recommend please an article where the mentioned protocol is giving the possibility to maximize the affinity to target sequence.
Is there any complex approach of LNA sequence customization/modification for different miRNAs of circRNAs?
Please, give advices how to reach high virus titer in cell culture after infection. Cultural liquid after this will be used to next infections and i need advices or protocols to achieve high...
22 April 2024 8,193 2 View
Dear Colleagues, We want to use microsatellite data to analyze the historical dynamics of mammalian populations and, in particular, to test the method of coalescent Bayesian scaling. We used...
17 April 2024 6,968 0 View
The term "ghost guns" refers to homemade or unregulated firearms, often using untraced components such as 3D-printed parts, assembly kits or replicas of existing firearm parts. These weapons are...
03 November 2023 6,920 0 View
Dear Colleagues, There is a famous formula of replacing variables in a stochastic differential equation (SDE), so called Itô's lemma. If we have SDE dx = a(x,t) dt + b(x,t) δW then the...
19 June 2023 983 1 View
Hello! We pump the contour of a metal tube with a diameter of 3 mm with oil with a viscosity of 0.0031 kg / m * s (abt. the viscosity is 4 times higher than that of water). We are interested in...
30 May 2023 3,596 8 View
Hello All! Why is it important to determine the coefficient of friction 𝜆 when determining hydraulic resistance? How important is it for the world to find a theoretical solution 𝜆 for a turbulent...
15 May 2023 3,146 3 View
Dear ResearchGate Community, I am currently conducting research in the field of algebraic geometry, and I am in need of the Magma (computer algebra system) for my analysis. Unfortunately, my...
22 April 2023 8,031 1 View
Hello all! I found an interesting relationship for convection coefficient for pipe fluid flow. Source: https://patents.google.com/patent/RU2600658C2/ru Please tell me, to what extent this...
27 February 2023 6,857 5 View
I am reading a book and it is quite old. It says that when determining hydraulic the friction coefficient λ for the turbulent regime zone, no theoretical solution to the problem was found. Is it...
19 October 2022 611 3 View
I am studying Wang W. "Reverse engineering". In FIG. 2.3 shows a block diagram. I don't understand why the laser beam passing through the lens has to bounce off the mirror before hitting the...
19 October 2022 9,150 0 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...
24 July 2024 8,696 0 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View
How to calculate percentage cumulative drug release when, Total volume of dissolution media = 250ml Added formulation was equivalent to 10mg of drug Sample withdrawn = 1ml each time Sink condition...
15 July 2024 4,192 1 View
Hi, In the 6 well plate, when I am seeding the cells. Do I add 1ml of complete media to one of the wells for the control at this stage or do I wait unit I am treating the plates to do that? Also...
13 July 2024 9,783 1 View
In my country, there is a lot of public outcry in relation to the presence of drug injection and related behaviors in public. Hence at least the bigger cities would probably benefit from the...
08 July 2024 8,903 1 View