Can i use Tween-20 instead NP-40/Triton X-100 for separation of cytoplasmic and nuclear RNA?

Hi there. I want to extract separately cytoplasmic and nuclear RNA fraction from leukemic cell line by Trizol. Most of protocols that I saw suggests using a buffer with NP-40 or Triton X-100 for...

07 September 2019 2,461 3 View