Oscar Recasens

7 Questions 42 Answers 0 Followers

Questions related from Oscar Recasens

Mutation found in the forward but not in the reverse sequence?

Hi! We are sequencing exon 5-6 from the BEST1 gene, we have multiple patients afected, with the explaining mutation, but in this cohort we found that in our forward sequences we can identify...

08 February 2024 2,316 5 View

Primers for STR´s?

Hi everyone and good morning! In our lab we want to make a sanger sequence opf the RPGR gene (ORF1/ NM_001034853.2) but is technically very dificult to make any PCR within that zones (it´s a...

28 September 2023 3,592 4 View

Protein Carbonylation detection protocol?

Hi everyone, and thanks in advance for reading! I want to determine oxidative stress human serum/plasma using protein damage; I´ve seen there are a lot of kits and methods, I want to try an ELISA...

12 January 2022 5,207 3 View

Prussian blue positive on fish spleen but not in Liver?

Hi everyone! I made a Perl's stain for some samples in my lab; they are old samples and I´m not sure how many time they spended on fixative (formol) or whatsoever, so I´m starting from the...

02 October 2020 6,713 3 View

Does anybody sonicated JVM2 cell Line for a transcription factor ChIP?

Hi!!! I´m going to sonicate a few millions of JVM2 for a trasncription factor ChIP; but since I never did that, I will have to check all conditions (crosslink time, sonication time, sonication...

26 August 2019 3,508 1 View

Higher DNA output after (kapa hyper prep kit) library amplification?

Hi, I tried 2 conditions on two different samples of different concentrations, and one sample gives an ultra concentration, I have an Idea of whats going on here, but I want more experimented...

14 June 2019 4,132 2 View

PCR works in some samples and does not in others...why?

Hello, and thanks for reading!! I´m using a 50mM NaOH solution for extraction (95ºC 30´) 2uL of DNA for reaction 1,25 uL primers 1uL MgCl2 0,5 uL of DNTP´S mix 0,25uL Taq to 23uL with sterile...

27 November 2018 4,527 8 View