Can i use Rpb2 Saccharomyces primers to identify other fungal species ?

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Linear Regression or correlation for OD and pH ?

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Is there any protocol describing how to make the measurement stress factors in plants (vegetal physiology)?

is there any protocol describing how to make the measurement stress factors in plants (vegetal physiology)?

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No title

When we use SD or SEM mean

01 January 1970 8,247 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

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02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

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Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

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What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

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28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

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26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

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25 February 2021 6,969 3 View

Genescan gives 3 peaks instead of one?

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QPCR reaction efficiency with odd results what does this indicate?

I am working on a CFX 96 machine to determine reaction efficiency of my gene of interest. After performing a serial dilution of my cDNA template, I was able to find great reaction efficiencies for...

23 February 2021 809 3 View

Alex Ignatov

These primers are identical in 70-80% (central part, except the ends) of length to homologous genes of other Eukaryotes. You can get target PCR products with other fungi using lower Ta, but you can get as well many non-specific bands.

Benjamin THOHA Thomas

following

Minhazur Rahman

yes, you can try with these. but ready to get some nonspecific band also.