Can I use RNA-later for viral isolation?

19 August 2022 1 4K Report

I'm planning to collect blood samples, oral and fecal swabs on the field from wild animals and I'm worried about sample preservation. I'd like to use RNA-later to preserve those materials but I also would like to use the same RNAlater preserved samples to possibly isolate viruses. However, I'm not sure If RNA-later is the best option to preserve virus particles from samples.

Keyla Santos Guedes de Sá

In my experience, it's much better to keep your samples on the trizol. The yield is much higher than the RNA latter, and the trizol samples also allow you to extract DNA and protein if you are interested in the future.

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Science method

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