Leonardo La Serra

13 Questions 16 Answers 0 Followers

Questions related from Leonardo La Serra

Can I use RNA-later for viral isolation?

I'm planning to collect blood samples, oral and fecal swabs on the field from wild animals and I'm worried about sample preservation. I'd like to use RNA-later to preserve those materials but I...

19 August 2022 3,691 1 View

How to titrate dengue virus?

Hello everyone, I'm trying to titrate different serotypes of dengue virus. I'm currently using VERO CCL81 cells in plaque assay and TCID50 assay, but only dengue serotype 2 virus showed good...

25 March 2022 1,165 1 View

How to check a pcr probe design?

I'm trying to check the primers and probe design from a paper. I'm using primer-BLAST to do so for the primers, but I'm not sure how to check the probe sequences.

12 February 2022 5,785 6 View

Any centrifugation assay for arbovirus isolation?

I'm trying to improve a centrifugation assay for arbovirus isolation using T75 flasks in VERO/C636 monolayers in order to yield the chances of virus isolation. I've tried a modified assay based on...

05 November 2020 7,724 0 View

Is anyone aware of a pan corona qPCR methodology approach?

I'm interested in protocols for a pan corona qPCR for human and non human samples.

06 April 2020 1,654 0 View

How long is a RNA stable enough to be used for RT-PCR, after extraction from different biological samples?

I have RNA samples stored at -70º, that have been extracted from animal blood, excrements, urine and saliva about 3 years ago. I wonder if this RNA samples are stable to use in a RT-PCR assay.

27 February 2017 3,720 6 View

Does anyone works with susceptibility bullous pemphigoid's HLA or know anything about the subject?

I'm currently working with blood biomarkers related to bulls pemphigoid. I could not find papers or works related to this subject.

17 December 2014 5,153 4 View

Does anyone know a good source of information about multivariable analysis?

I'm working with a table of demographic information about my patients and controls, such as job information, whether or not they have been exposed to heavy metal, to pesticides or to any water...

11 December 2014 747 9 View

Does anyone know a good article about the dynamic of pesticides and heavy metals in the environment?

I'm working with pesticides and heavy metals in water and I'm gathering as much information about it as possible.

22 March 2014 6,503 4 View

Does anyone know of studies relating pesticides and heavy metals to the bullous disease pemphigus?

I'm working with the triggering relation between heavy metals and organophosphorus pesticides with the skin disease called pemphigus.

31 October 2013 1,826 14 View

Does anyone have statistics to help me to create a control group?

I need to create a representative control group of blood donors to establish the concentration of Pb for my city's population.

03 August 2013 3,206 1 View

Does anyone know any methods to collect and measure heavy metals and pesticides in blood and water?

It's actually a part of my masters to measure heavy metals, organochlorine and organophosphate in stream water and blood, but I've been having trouble finding methods to do it.

28 July 2013 8,720 2 View

What´s the regular viremia for the main flavivirus and alphavirus in human?

I´m currently working with the viability of virus infection in blood bags from regular blood donors. Hence, I´m wondering what would be the most realistic form to mimic a real infection by a...

01 January 1970 2,472 5 View