Leonardo La Serra

7 Questions 10 Answers 0 Followers

Questions related from Leonardo La Serra

Can I use RNA-later for viral isolation?

I'm planning to collect blood samples, oral and fecal swabs on the field from wild animals and I'm worried about sample preservation. I'd like to use RNA-later to preserve those materials but I...

19 August 2022 3,681 1 View

How to titrate dengue virus?

Hello everyone, I'm trying to titrate different serotypes of dengue virus. I'm currently using VERO CCL81 cells in plaque assay and TCID50 assay, but only dengue serotype 2 virus showed good...

25 March 2022 1,157 1 View

How to check a pcr probe design?

I'm trying to check the primers and probe design from a paper. I'm using primer-BLAST to do so for the primers, but I'm not sure how to check the probe sequences.

12 February 2022 5,772 6 View

Is anyone aware of a pan corona qPCR methodology approach?

I'm interested in protocols for a pan corona qPCR for human and non human samples.

06 April 2020 1,640 0 View

How long is a RNA stable enough to be used for RT-PCR, after extraction from different biological samples?

I have RNA samples stored at -70º, that have been extracted from animal blood, excrements, urine and saliva about 3 years ago. I wonder if this RNA samples are stable to use in a RT-PCR assay.

27 February 2017 3,705 6 View

Does anyone know of studies relating pesticides and heavy metals to the bullous disease pemphigus?

I'm working with the triggering relation between heavy metals and organophosphorus pesticides with the skin disease called pemphigus.

31 October 2013 1,812 14 View

Does anyone have statistics to help me to create a control group?

I need to create a representative control group of blood donors to establish the concentration of Pb for my city's population.

03 August 2013 3,191 1 View