01 January 2017 4 3K Report

I order the polyfect transfection  reagent (Cat No./ID: 301105) . can use it for transfection of mimic mirna? 

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Why DNA skein and DNA pellet were not seen during salting out DNA extraction method?

I use salting out DNA extraction methods . But recently after adding the ethanol we couldn't saw the DNA skein and DNA pellet . Finally the results of nano drop indicated that extracted DNA have...

08 September 2019 2,017 1 View

How can i extract miRNa from saliva samples efficiently ?

Recently we decide to evaluate the miRNA expression levels in saliva sample as diagnostic biomarker of cancer . We used TRIzol extraction methods but the CT of real-time PCR for U6 gene was...

08 September 2019 1,548 3 View

What is the serum IL-6 level in human ?

Recently we evaluate the serum IL-6 level in patients after radiotherapy . Unfortunately 4 of 7 samples are negative. we used the thermo fisher ELISA kit for this aim and 100 microliter of...

07 August 2019 2,793 5 View

Is there differences between PBMC and WBC in ionizing radiation responses?

I want to evaluate the ionizing radiation effects on miRNA expression level in mice. Some paper used  PBMCs  and others used whole blood ( WBC ) for RNA extraction .  is there difference between...

04 May 2019 8,140 5 View

How can i access the Miranda web page ?

unfortunately during this month i couldn't access to the miranda microRNA target prediction tool web page . http://www.microrna.org/ is the web page address was changed ?

05 June 2018 9,454 0 View

How can i access to the LncRNA sequences?

I want to design primer for the lncRNA entitled nonhsat062994 but i can't find it at NCBI. how can i access to the sequence of this RNA?

04 May 2018 8,787 4 View

How can i solve the agarose gel problem ?

My friend sent me the agarose gel results. As seen in attached figure lower range of gel was dark and , i cant see any band in this region . how can i solve this problem?

11 December 2017 5,031 0 View

How can I extract DNAparaffin embedded tissues with mineral oil?

Recently two study indicated that mineral oil can be used for Dna extraction. Is there anyone who has the experience about this method?

10 November 2017 4,348 5 View

How can i predict that targeting a gene with siRNA lead to specific pathway inhibition ?

Recently we want to target a gene with siRNA , but i have a critical question how can i make sure that downstream pathway of this genes will be affected . on the other hand ho can i predict...

10 November 2017 2,047 3 View

Why the lan of sds page aren't smoth?

I run the sds page but after transfer to membran and staining I saw that lower band are biger than higher band.  How can i solve it?

08 September 2017 7,942 3 View

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