unfortunately during this month i couldn't access to the miranda microRNA target prediction tool web page . http://www.microrna.org/
is the web page address was changed ?
I use salting out DNA extraction methods . But recently after adding the ethanol we couldn't saw the DNA skein and DNA pellet . Finally the results of nano drop indicated that extracted DNA have...
08 September 2019 2,017 1 View
Recently we decide to evaluate the miRNA expression levels in saliva sample as diagnostic biomarker of cancer . We used TRIzol extraction methods but the CT of real-time PCR for U6 gene was...
08 September 2019 1,548 3 View
Recently we evaluate the serum IL-6 level in patients after radiotherapy . Unfortunately 4 of 7 samples are negative. we used the thermo fisher ELISA kit for this aim and 100 microliter of...
07 August 2019 2,793 5 View
I want to evaluate the ionizing radiation effects on miRNA expression level in mice. Some paper used PBMCs and others used whole blood ( WBC ) for RNA extraction . is there difference between...
04 May 2019 8,140 5 View
I want to design primer for the lncRNA entitled nonhsat062994 but i can't find it at NCBI. how can i access to the sequence of this RNA?
04 May 2018 8,787 4 View
My friend sent me the agarose gel results. As seen in attached figure lower range of gel was dark and , i cant see any band in this region . how can i solve this problem?
11 December 2017 5,031 0 View
Recently two study indicated that mineral oil can be used for Dna extraction. Is there anyone who has the experience about this method?
10 November 2017 4,348 5 View
Recently we want to target a gene with siRNA , but i have a critical question how can i make sure that downstream pathway of this genes will be affected . on the other hand ho can i predict...
10 November 2017 2,047 3 View
I run the sds page but after transfer to membran and staining I saw that lower band are biger than higher band. How can i solve it?
08 September 2017 7,942 3 View
Recently ,we decide to extract cancer stem cell from primmery cancer cells. for this aim can we use the non-treated flask. on the other hand what is the difference of non-treated flask with...
07 August 2017 1,493 4 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...
24 June 2024 3,507 2 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...
16 June 2024 9,723 2 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
06 June 2024 5,715 4 View
The size miRNA is 89 bps and the primers I have designed (using traditional method for designing gene primers) should give an amplified product of 71 bps. But I am getting product of higher...
23 May 2024 7,570 3 View
I have a question regarding target miRNA expression after transfection with antisense miRNA (miRNA inhibitor). I understand that the expression of the target miRNA will increase after mimic...
20 May 2024 5,920 4 View
Hello. Is there any webserver where I can input a set of transcription factors, genes, and miRNAs, and it can provide me with statistical data on how TFs either activate or repress genes and...
18 May 2024 5,848 1 View
Hello all. I have recently been trying to isolate total RNA after transfection of miRNA mimics and antisense inhibitors in mammalian cells. However, the RNA isolated through the trizol method...
13 May 2024 4,935 7 View
would like to work on microRNA to detect colorectal cancer. I will use the type of microRNA type micRNA124,micro143 how I can make primer design for it and how I can choose the primer ?
07 May 2024 8,812 2 View