Small displacement measurement in vacuum?

Hello, I have a question. there is a circular thin foil which vibrates in vacuum, the foil restricted and moves up and down. it is like a diaphragm between two pipes . both sides are in vacuum but...

18 February 2021 8,075 3 View

I have a problem with sensible heat flux (H) iteration process in Sebal model?

In each iteration, I get the same value of H for different values of aerodynamic resistance and the temperature difference between two heights. Do the results make sense? Is it necessary to adjust...

07 February 2021 8,953 2 View

What is the best book to teach entrepreneurship to primary students?

What is the best book to teach entrepreneurship to primary students?

07 September 2020 6,757 3 View

Which algorithm (Sobel, Perwitt, Roberts, Canny and LOG algorithm) is more capable of finding noisy image edges?

The edge detection algorithms are designed to respond to sharp edges which can be caused by noisy pixels. In image analysis edge detection plays an important role and it is one of the traditional...

05 August 2020 2,543 1 View

Internal pressure of CT scan x-ray vacuum insert ?

Hello,, Dose anyone know the internal pressure of x-ray vacuum insert used in CT scan machines? Thank you

03 August 2020 8,748 2 View

Which source do you advise to learn Ansys Fluent?

Dear Colleagues, I am currently working on a new project which needs to be modeled by ANSYS FLUENT. As you know, there are many sources including tutorials, online courses, books, etc. However, I...

11 July 2020 2,344 10 View

What is the best way of Evaluation Performance for Multiclass Data?

For classification problems, classifier performance is typically defined according to the confusion matrix associated with the classifier. Based on the entries of the matrix, it is possible to...

19 May 2020 3,472 4 View

Best EEG dataset for sleep stage classification?

I am looking for a best dataset with signals of EEG that is used for sleep stage classification.

12 May 2020 5,935 3 View

What are the public biosignals (ECG, etc) datasets of COVID-19 patients?

I am researching QOVI-19 and need a database that contains biosignal data such as electrocardiograms. Is there any dataset?

12 May 2020 9,274 3 View

How can I download FDA approved drugs from zinc database ?

Recently I want to download the FDA approved drugs but unfortunately I couldn't retrieve data correctly . How can I download this data in SDF format?

28 March 2020 3,785 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View