Hi,
I have been trying to isolate metagenomic DNA using the conventional method of DNA extraction by using SDS for cell lysis and Isopropanol for precipitating the DNA followed by 70% ethanol wash. The resultant DNA contained humic acid contamination.
To avoid such contamination I was suggested to use PEG 6000, because of the non-availability of PEG 6000 can I use PEG 4000. If so how can I compensate PEG 6000 with PEG 4000?
Kindly help me in this regard.
Thank you in advance for your answers.
I think it's worth trying. Generally, PEG 8000 is considered effective for DNA precipitation in case of humic acid contamination, but PEG 6000 and 4000 can also be used. They all can show DNA precipitating properties, but have differences in their molecular weights and efficiency. You may increase the concentration of PEG 4000 to compensate for its molecular weight.
- Badges
- Science topic