Hello all,

my question is about using my frozen samples to detect macrophages. I have uterine cells, scratched with 40um strainer and frozen in -81 degrees in 90% FBS and 10% DMSO medium. They was centrifuged with 1500 rcf. My question is, can I use this samples to detect macrophages? It will be very usefull for me to mark them in this samples for flow cytometry, but I dont really know if it have any sense since this samples was frozen for other purposes and it is propably not most optimal way to freeze it for macrophages.

Thank you for all answers,

Szymon

Similar questions and discussions