Can i use miRNeasy serum/plasma cel-39-3p spike in instead of U6 snRNA as control in qRT-PCR for miRNA of endothelial cell origin?

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No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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MiRNA qpcr problem?

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Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

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Amanda Salviano-Silva

Yes, you can. Specially if you are quantificating RNA levels of extracellular vesicles or other sources with high levels of variantion in gene expression.

Paroma Deb

I am going to see miRna fold changes in treated and untreated sample of endothelial cells. So if i take exogenous control cel 39 ,should i use it as housekeeping gene for double delta CT method for analysis?

If you could please explain me how to analyze this ,it'll be very helpful..Thanks in advance!@ Amanda Salviano-Silva, Cesar Augusto Sam Tiago Vilanova-Costa