Yes, you can. Specially if you are quantificating RNA levels of extracellular vesicles or other sources with high levels of variantion in gene expression.
I am going to see miRna fold changes in treated and untreated sample of endothelial cells. So if i take exogenous control cel 39 ,should i use it as housekeeping gene for double delta CT method for analysis?
If you could please explain me how to analyze this ,it'll be very helpful..Thanks in advance!@ Amanda Salviano-Silva, Cesar Augusto Sam Tiago Vilanova-Costa