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Questions related from Paroma Deb
I have coated my culture flask with more concentrated gelatin than required accidentally. I haven't seeded my cells yet. Can i uncoat them and again coat them with desired concentration?
25 April 2019 657 1 View
I am going to do immunofluorescence from my cultured HUVEC. Before doing IF test i need to treat my cells with my test serum sample for 6 hours. I don't have chamber slide for this. So I am in...
09 April 2019 4,071 3 View
I wanna analysis cytokines level in different stages of CIN and ca cervix both in HPV DNA positive and negative cases. We collected 1000microliter cervical swab by cyto brush for detection of HPV...
13 March 2019 2,734 2 View
I dont have enough time to order for U6 snRNA,so what can i do instead? Please help me out!
28 November 2018 3,129 4 View
I intend to see miR expression in Endothelial cells from HUVEC. I have some remaining miRNeasy serum/plasma spike in control cel-39-3p from another experiment. I am thinking to use it as qPCR...
11 November 2018 6,078 0 View
I am going to do endothelial cell culture from HUVEC. Then i will see several miRNA expression on them after treating endothelial cells with virus infected sera. Right now i dont have my culture...
31 October 2018 1,455 4 View
For my MD thesis purpose I am going to see endothelial permeability change after treating them with Dengue infected patients sera. I will establish endothelial cell line from HUVEC. In most of...
11 August 2018 7,855 5 View
I am planning to establish primary endothelial cell culture from HUVEC and then to treat them with Dengue virus infected serum for further experiment. I need to confirm my cultured cell as...
24 June 2018 841 5 View