Paroma Deb

8 Questions 8 Answers 0 Followers

Questions related from Paroma Deb

Can i uncoat my gelatin from cell culture flask?

I have coated my culture flask with more concentrated gelatin than required accidentally. I haven't seeded my cells yet. Can i uncoat them and again coat them with desired concentration?

25 April 2019 653 1 View

Can i do immunofluorescence in cover slip for my HUVEC? If so, how?

I am going to do immunofluorescence from my cultured HUVEC. Before doing IF test i need to treat my cells with my test serum sample for 6 hours. I don't have chamber slide for this. So I am in...

09 April 2019 4,069 3 View

How to preserve and process endocervical swab for cytokin analysis by flow cytometry?

I wanna analysis cytokines level in different stages of CIN and ca cervix both in HPV DNA positive and negative cases. We collected 1000microliter cervical swab by cyto brush for detection of HPV...

13 March 2019 2,732 2 View

Can i use miRNeasy serum/plasma cel-39-3p spike in instead of U6 snRNA as control in qRT-PCR for miRNA of endothelial cell origin?

I dont have enough time to order for U6 snRNA,so what can i do instead? Please help me out!

28 November 2018 3,128 4 View

Can i use miRNeasy serum/plasma spike in control qPCR normalization kit (QIAGEN) for my EC sample obtained from HUVEC ?

I intend to see miR expression in Endothelial cells from HUVEC. I have some remaining miRNeasy serum/plasma spike in control cel-39-3p from another experiment. I am thinking to use it as qPCR...

11 November 2018 6,077 0 View

Is miRNA expression level in lymphocyte is same as endothelial cells?

I am going to do endothelial cell culture from HUVEC. Then i will see several miRNA expression on them after treating endothelial cells with virus infected sera. Right now i dont have my culture...

31 October 2018 1,454 4 View

For assessing endothelial permeability change if i go for only VE-cadherin expression study in cell culture will it be appropriate?

For my MD thesis purpose I am going to see endothelial permeability change after treating them with Dengue infected patients sera. I will establish endothelial cell line from HUVEC. In most of...

11 August 2018 7,854 5 View

What are the methods for confirming cultured cells from HUVEC as endothelial cell & which one is most appropriate?

I am planning to establish primary endothelial cell culture from HUVEC and then to treat them with Dengue virus infected serum for further experiment. I need to confirm my cultured cell as...

24 June 2018 838 5 View