I aim to measure the concentration of hydrocarbon based organic compound from a certain quantity of soil sample. I would really appreciate it if anyone can give me some experimental guidelines.
To measure paraffin oil or hydrocarbons in a soil sample:
1. Extract hydrocarbons from the soil using solvents like hexane.
2. Purify the extract using methods like column chromatography.
3. Analyze purified samples using gas chromatography (GC) or high-performance liquid chromatography (HPLC) with appropriate detectors.
4. Create calibration standards to quantify hydrocarbon concentrations.
5. Compare sample peaks to calibration curves to determine hydrocarbon levels in the soil.
Include quality control measures for accuracy and reliability.
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