Can delta Ct value present as negative results?

I have studied miRNA expression by calculating 2-ddCt . After I have performed qRT-PCR of target and reference miRNAs (hsa-miR-16-5p), I calculate delta Ct by using this formular " Ct(target...

07 August 2018 7,365 3 View

Question about how to determine miRNA concentration ?

I am working with miRNA expression and diseases. I want to know about the protocols for miRNA quantification. Can I use NanoDrop spectrophotometry for miRNA quantification?

02 March 2018 5,203 1 View

Can I use 2^-dCT to determine relative gene expression between before and after treatment in each patient?

I have studied different gene expression in patients before and after treatment. Can I use 2^-dCT in each time point to compare the different gene expression in our patients? I think if I...

04 May 2016 5,564 8 View

What is the suitable specimen for ELISA test, Plasma or serum?

I have done ELISA test to determine the level of cytokine in blood. There are many studies suggesting that either plasma or serum can be used for the assay. I would like to discuss with all of you...

01 February 2016 6,540 7 View

What is the appropriate volume of serum/plasma for mRNA extraction ?

I plan to conduct my research about gene expression by detecting mRNA of interested gene in serum/plasma. Due to the limitation volume of collected and divided serum/plasma, I would like to...

31 December 2015 4,448 6 View

Could you tell me how to calculate the SNPs results with OR and compare the genotypic frequency of each SNPs type with SPSS?

I have done the project about genetic polymorphism and now I face some problem about how to use the statistical analysis to evaluate my results. Could you give me some suggestion for SNPs...

10 November 2015 9,384 7 View

What is the best protocol for DNA extraction, manual or commercial kit?

For DNA extraction from cell line or PBMCs, which protocol is suitable for this experiment? In my opinion, commercial kit such as DNA extraction kit with silica column is convenience and providing...

08 September 2015 3,321 9 View

The suitable endogenous miRNA for the study of circulating miRNA by qRT-PCR

I have conduct the research about circulating miRNA detection in infectious diseases by qRT-PCR. I have some points to discuss with all of you as follows: 1. I have studied miRNA expression in...

01 January 1970 7,906 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View