Methee Sriprapun

9 Questions 9 Answers 0 Followers

Questions related from Methee Sriprapun

Can delta Ct value present as negative results?

I have studied miRNA expression by calculating 2-ddCt . After I have performed qRT-PCR of target and reference miRNAs (hsa-miR-16-5p), I calculate delta Ct by using this formular " Ct(target...

08 August 2018 7,377 3 View

Question about how to determine miRNA concentration ?

I am working with miRNA expression and diseases. I want to know about the protocols for miRNA quantification. Can I use NanoDrop spectrophotometry for miRNA quantification?

03 March 2018 5,218 1 View

Can I use miRNA expression of adults as the control groups compare to miRNA expression in children?

I have conduct the research about miRNA expression in infectious disease in children. I want to compare results with control group but the problem is about the difficult to obtain blood from...

06 June 2017 8,408 1 View

Can I use 2^-dCT to determine relative gene expression between before and after treatment in each patient?

I have studied different gene expression in patients before and after treatment. Can I use 2^-dCT in each time point to compare the different gene expression in our patients? I think if I...

05 May 2016 5,576 8 View

What is the suitable specimen for ELISA test, Plasma or serum?

I have done ELISA test to determine the level of cytokine in blood. There are many studies suggesting that either plasma or serum can be used for the assay. I would like to discuss with all of you...

02 February 2016 6,553 7 View

What is the appropriate volume of serum/plasma for mRNA extraction ?

I plan to conduct my research about gene expression by detecting mRNA of interested gene in serum/plasma. Due to the limitation volume of collected and divided serum/plasma, I would like to...

01 January 2016 4,462 6 View

Could you tell me how to calculate the SNPs results with OR and compare the genotypic frequency of each SNPs type with SPSS?

I have done the project about genetic polymorphism and now I face some problem about how to use the statistical analysis to evaluate my results. Could you give me some suggestion for SNPs...

11 November 2015 9,397 7 View

What is the best protocol for DNA extraction, manual or commercial kit?

For DNA extraction from cell line or PBMCs, which protocol is suitable for this experiment? In my opinion, commercial kit such as DNA extraction kit with silica column is convenience and providing...

09 September 2015 3,338 9 View

The suitable endogenous miRNA for the study of circulating miRNA by qRT-PCR

I have conduct the research about circulating miRNA detection in infectious diseases by qRT-PCR. I have some points to discuss with all of you as follows: 1. I have studied miRNA expression in...

01 January 1970 7,921 5 View