How to find statistical difference between category counts in a multiple-response survey question?

Which statistical test is appropriate if we want to look for statistical differences between total counts for each category, if the subjects can select multiple categories in their response? The...

23 January 2021 6,926 3 View

Is it possible to add hierarchical porous Cu/Zn-nanoparticle@carbon materials into a carbon paper based gas diffusion electrode (GDE) ?

26 November 2020 5,075 1 View

Tips for students on how to take care of a work-life balance during a covid19 pandemic ?

Students asked me for advice on how to deal with work-life balance during a lockdown. Some of them work and study from home. Do you have any helpful suggestions? I found an article that suggests...

29 October 2020 5,994 2 View

Best tips for engaging students during remote lectures ?

The academic year has begun, and at least for now it will be coordinated remotely. What ways do you have to engage students' attention during remote lectures? I was thinking about asking...

04 October 2020 9,988 38 View

How do I create a matrix file from my variant frequency to create a sequence logo?

I would like to create a sequence logo from my variant frequency (short 15bp DNA sequence). So it's not the normal alignment of several sequences but only ONE single sequence where I would like to...

31 March 2020 1,405 8 View

Protocol: Single-Locus and Multilocus DNA Fingerpriting, Bruford et al. 1992?

Hi everyone! I know this probably isn't the right place for this, but I have exhausted all other options to access this paper. Does anyone has a pdf copy of the chapter "Single-Locus and...

25 March 2020 2,540 5 View

Can individual, dyadic and triadic interviews be analyzed together?

I am conducting a multiperspective interviews on cultural competence in healthcare institutions (management level). Some interviews are individual, but in some institutions, they expressed the...

03 May 2019 4,387 4 View

How can I reduce the diameter of a ceramic membrane support?

I have a flat support of 6 cm (Al2O3 obtained by dry pressing) and need to reduce it to 3.8 cm for test in the gas permeation module. The rsintering shrinkage was not enough, how could I cut this...

12 November 2018 7,993 2 View

RNAlater reaction with PBS?

Currently planning to make hookworm protein extract with frozen adult hookworms in RNAlater solution. I am planning to first remove all of the RNAlater and wash in 1X PBS and eventually gauge...

15 July 2018 7,075 1 View

Flow Cytometry protocol for Tregs detection (CD4+, CD25high, FoxP3+) in fresh isolated horses PBMCs ?

Hello, I'm Trying to mark Tregs population in Horses, I adapted a protocol used to mark Tregs in dogs, and It worked at the beginning. But after 7 months I started my experiment and I haven't...

25 May 2018 7,953 5 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View