I think that it Works quite well no problem

Hello

Agree with researchers  you can use without problem , Also . find links here will help you

Good luck

http://www.hek293.com/transfection-information/

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3338230/

http://compbio.dfci.harvard.edu/compbio/research/labprotocols/contentBlocks/0/contentBlock_files/file15/Lentivirus_Production_and_Transfection_Protocol.pdf

http://resslerlab.com/site/wp-content/uploads/2012/10/Lentivirus.doc

Hi Ebr Bio,

Yes you can! You can follow our routine lentivirus infection protocol to establish your HEK293 stable cell line. 

https://www.abmgood.com/Documents/files/Full-A4-lenti-infection.pdf

Good Luck!

Hi.this vector and host are exactly match.

Dear esteemed colleague,

I hope this message finds you well and making significant progress in your research. Your inquiry about the use of lentivirus for transfecting HEK293 cells to establish a stable cell line is indeed a pertinent strategy within molecular biology and genetic engineering fields. The answer is unequivocally affirmative; lentiviral vectors are extensively utilized for this purpose due to their high efficiency in transducing a wide variety of cell types, including HEK293 cells.

1. Advantages of Using Lentivirus:

• High Transduction Efficiency: Lentiviruses can efficiently infect both dividing and non-dividing cells, making them highly versatile for various experimental needs.
• Stable Integration: The lentiviral genome integrates into the host cell genome, allowing for stable expression of the transgene in the cell line over an extended period, through numerous cell divisions.
• Broad Host Range: Lentiviruses are capable of transducing a wide range of cell types, including difficult-to-transfect primary cells and stem cells.

2. Key Considerations for Lentiviral Transfection:

• Vector Design: Select a lentiviral vector that includes your gene of interest, a strong promoter to drive expression, and a selection marker (such as puromycin resistance) to facilitate the identification and selection of successfully transduced cells.
• Viral Production and Titration: Produce lentivirus in packaging cells (often HEK293T) and then titrate the viral particles to determine the optimal multiplicity of infection (MOI) for your transduction experiment.
• Safety Precautions: Given that lentivirus is a derivative of HIV, although engineered to be replication-incompetent for safety, it is essential to follow biosafety level 2 (BSL-2) practices when handling and disposing of lentiviral preparations.

3. Protocol Overview for Creating Stable HEK293 Cell Lines:

4. Conclusion:

Utilizing lentivirus to transfect HEK293 cells for the creation of a stable cell line is a proven and effective methodology. By following carefully designed protocols and adhering to safety guidelines, you can achieve stable, long-term expression of your gene of interest for various research applications.

Should you require further assistance or wish to delve deeper into any specific aspect of your project, please do not hesitate to reach out.

Warm regards,

With this protocol list, we might find more ways to solve this problem.