Dear Al,
I am working on WB, and looking for ECL products with lower background and stronger signal. I have tried the GE-ECL primer but it turned out to be not ideal.
can anyone suggest me some better product?
Please check the link.
https://www.thermofisher.com/order/catalog/product/32106
You can use Millipore's Cresendo western HRP substrate. Its the best one i have used.
Try this ECL kit from Biorad.
http://www.bio-rad.com/en-au/product/clarity-western-ecl-blotting-substrate.
Make sure you block your membrane well to eliminate as much background as possible.
Good luck!
Thanks for all your sugestions and I will try and see.
Chromosome/DNA/Molecular Biology
05 December 2022 3,563 2 View
I have a tabular dataset made of floats which follow a simple rule like: "if features A and B are in a certain range then target class is 1, otherwise target class is 0." Since I want to get...
04 May 2021 3,983 0 View
I'm trying to generate the interpretability of a large dataset with 177000 features. Specifically I am doing permutation using the permutation_importance method from scikit-learn. I'm using a...
15 April 2021 8,621 0 View
Hello, In part of my project, i have to extract cell nucleus after cells lysis in order to measure nuclear radioactivity and also cytoplasm+membrane fraction radioactivity. For this, it is...
03 January 2021 9,042 3 View
Feeding knowledge directly into your brain, just like in sci-fi classic The Matrix, by a simulator which can feed information directly into a person’s brain and teach them new skills in a shorter...
07 March 2018 1,479 3 View
Whether the method is specific for different species? if so kindly say the method used for counting dormant cells of sulphate reducing bacteria (SRB)
12 March 2013 8,958 3 View
Can anyone suggest where I can get for a given Microgrid data such as · Electrical load; · Thermal load (cooling/heating); · Grid energy price; · Grid energy supply availability; · Renewable...
01 January 1970 9,961 4 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View